Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.215993 |
Chromosome: | chromosome_14 |
Location: | 2452237 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g624950 | DHC10,IDA2,DYH1B | Flagellar inner arm dynein 1 heavy chain beta | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTAGGATGCCAGCACGCGGCGGTCCAGCTC |
Internal bar code: | AATTTTCCTACCGTCTGGCAGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 954 |
LEAP-Seq percent confirming: | 99.6916 |
LEAP-Seq n confirming: | 9374 |
LEAP-Seq n nonconfirming: | 29 |
LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTCCCAGCATACCCAGTGT |
Suggested primer 2: | ATAGCGTGTCAAAAGTGGGG |