Insertion junction: LMJ.RY0402.215995_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.215995
Chromosome:chromosome 11
Location:2471828
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g475600 FAP362 Flagellar Associated Protein 362; (1 of 239) IPR016024 - Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTATGGTTCTGCAGACGGTCTATACATCA
Internal bar code:TACTAAGCGACCTTCATCAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:892
LEAP-Seq percent confirming:99.5227
LEAP-Seq n confirming:16891
LEAP-Seq n nonconfirming:81
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CTCAATCTGCCGGATCTTGT
Suggested primer 2:CAAGCTGGCGTATGTCATTG