| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216009 |
| Chromosome: | chromosome 16 |
| Location: | 2389114 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g660100 | (1 of 19) IPR008984 - SMAD/FHA domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGCTTGCTGTGACCGTGTTCCCGGCAG |
| Internal bar code: | ACTGGGATAGCGGTTTGAAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 684 |
| LEAP-Seq percent confirming: | 99.1539 |
| LEAP-Seq n confirming: | 4453 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTGCTCTGGTGCTATGA |
| Suggested primer 2: | GAGGCCGTAATAGCAGCTTG |