| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.216071 |
| Chromosome: | chromosome 7 |
| Location: | 4981312 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346850 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTTCGGAGGACCCACGCACCGGGGATTG |
| Internal bar code: | ACGCGTGTACTAGTTCGGTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1098 |
| LEAP-Seq percent confirming: | 98.6318 |
| LEAP-Seq n confirming: | 3244 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTAGCCCTCTTACACGTC |
| Suggested primer 2: | GTTGGATATGGAAACACCCG |