| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.216081 |
| Chromosome: | chromosome 7 |
| Location: | 1548664 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g324222 | 3'UTR|intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAATAGCGTCACAAGCCAAAGCAGCCTG |
| Internal bar code: | CACTACGCCCTGGTTGGTGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 973 |
| LEAP-Seq percent confirming: | 99.2371 |
| LEAP-Seq n confirming: | 18080 |
| LEAP-Seq n nonconfirming: | 139 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGGTACCGTGATAGCAG |
| Suggested primer 2: | CTTTGGGTTGCTACTGCCAT |