| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.216082 |
| Chromosome: | chromosome 9 |
| Location: | 5165339 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399104 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAGGAAGTGTTCAGCAATTGTTTGCCAA |
| Internal bar code: | ATTTGCCCGCGAAGGAATGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 954 |
| LEAP-Seq percent confirming: | 86.0926 |
| LEAP-Seq n confirming: | 8258 |
| LEAP-Seq n nonconfirming: | 1334 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTAGACCTGCTTTCGGTGC |
| Suggested primer 2: | CATACGCACACACACAGCAG |