| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.216084 |
| Chromosome: | chromosome 12 |
| Location: | 150795 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g484400 | FKM7 | DNA methylase in the FkbM family; (1 of 13) PF05050 - Methyltransferase FkbM domain (Methyltransf_21) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGTGACACGGGTGCAATTCGGCCTGGC |
| Internal bar code: | TGTAGGACCGGGAGGATTCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1129 |
| LEAP-Seq percent confirming: | 99.4719 |
| LEAP-Seq n confirming: | 12432 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTATTGACGTGTGTTGCGG |
| Suggested primer 2: | GTTTACGGTTTAGGTCCGCA |