| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.216089 |
| Chromosome: | chromosome 6 |
| Location: | 4983647 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279950 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCACCCGCGCGGCCGCAGATCTCGGAT |
| Internal bar code: | GGCTTCGGGTTGACGGTAAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1005 |
| LEAP-Seq percent confirming: | 99.7238 |
| LEAP-Seq n confirming: | 22023 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTGCAGTGCACAAGGACC |
| Suggested primer 2: | AAGGCAATACCGTATCGTGG |