Insertion junction: LMJ.RY0402.216096_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.216096
Chromosome:chromosome 2
Location:2717640
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g093600 TEH3 (1 of 6) PF03061 - Thioesterase superfamily (4HBT); Thioesterase-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACAGCGCGCGGCCGCGGGCGTACACAACC
Internal bar code:CAGGGACCAATTAGCGCCCACG

Confirmation - LEAP-Seq

LEAP-Seq distance:788
LEAP-Seq percent confirming:98.0747
LEAP-Seq n confirming:4279
LEAP-Seq n nonconfirming:84
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:ACACCCACCTATCCAAGCAG
Suggested primer 2:GCCCATGCACTCCTACAGTT