Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.216102 |
Chromosome: | chromosome_1 |
Location: | 6675806 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g047950 | FAP156,IFT27 | Intraflagellar transport protein 27 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCCTAAGCAAATACTAGACTTCACCGGCT |
Internal bar code: | AAAAAATAGAGTGGTCAAGGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 783 |
LEAP-Seq percent confirming: | 99.5624 |
LEAP-Seq n confirming: | 19566 |
LEAP-Seq n nonconfirming: | 86 |
LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCTCGTGTTCGATGTGAGC |
Suggested primer 2: | CGTTTGTTGCGGTGTATGAC |