| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216102 |
| Chromosome: | chromosome_1 |
| Location: | 6676027 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g047950 | FAP156,IFT27 | Intraflagellar transport protein 27 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCGGCGTGTCTGCTTATGTAATGATGTTT |
| Internal bar code: | TCGGTGGAATCTGGGGAGCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAACACCCTCGACTTCT |
| Suggested primer 2: | GTGAACCAATCGGAGCAAAT |