| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.216123 |
| Chromosome: | chromosome 12 |
| Location: | 9532448 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544327 | (1 of 37) PF13639 - Ring finger domain (zf-RING_2) | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAGGTGGGAACCCGGCAGTGCTAGAAG |
| Internal bar code: | CGTATTTGTCCTCAGCGACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 239 |
| LEAP-Seq percent confirming: | 99.2038 |
| LEAP-Seq n confirming: | 623 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTTCCAAACCTGGATCTC |
| Suggested primer 2: | GCCCTAACATACAGGCTTGC |