Insertion junction: LMJ.RY0402.216173_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.216173
Chromosome:chromosome 2
Location:436072
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g076200 (1 of 1) 3.4.11.2 - Membrane alanyl aminopeptidase / Peptidase E CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCACCAGTGGTGAGGCAGGGGGTCATGTG
Internal bar code:TTTATGATATAAACGGTTGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:210
LEAP-Seq percent confirming:98.4556
LEAP-Seq n confirming:255
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTGACGTTCCAAACACCT
Suggested primer 2:GCTAGGGACCTGGTGTGTGT