| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216174 |
| Chromosome: | chromosome 7 |
| Location: | 1389725 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322650 | CSA1 | Chlorophyceae-specific gene family A protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGATGTAGCAAGCCTACCTAAGACTCC |
| Internal bar code: | TATCAATGCTGGACATCATGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 889 |
| LEAP-Seq percent confirming: | 99.3449 |
| LEAP-Seq n confirming: | 13345 |
| LEAP-Seq n nonconfirming: | 88 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTATCTAGCACGGCTTGG |
| Suggested primer 2: | TAAACCAAAGTGTGCTTGCG |