| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.216189 |
| Chromosome: | chromosome 10 |
| Location: | 3037478 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441250 | TMEM231 | (1 of 1) K19362 - transmembrane protein 231 (TMEM231); Transmembrane protein 231 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCCGTCCTTTGGTCCGTCTACAGGCATT |
| Internal bar code: | ATTACGAGATGCGGTCCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 889 |
| LEAP-Seq percent confirming: | 99.5652 |
| LEAP-Seq n confirming: | 229 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGTCCGTCAAACGTGTTC |
| Suggested primer 2: | AGCATTGTTCAGGGTTCAGG |