| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.216216 |
| Chromosome: | chromosome 2 |
| Location: | 2853922 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094750 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCCAGTAGCCAAACTCTGCAGAGCTTT |
| Internal bar code: | GGCTGGCACCAAGGATTACCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1059 |
| LEAP-Seq percent confirming: | 98.9194 |
| LEAP-Seq n confirming: | 2197 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGAACACCGGAGAGTGTC |
| Suggested primer 2: | GAATGTGGAGACGGAAGGAA |