| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216231 |
| Chromosome: | chromosome 5 |
| Location: | 907408 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g247350 | (1 of 30) IPR000253 - Forkhead-associated (FHA) domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGAATGCAGGCACGTAGGAAACAAGGG |
| Internal bar code: | ACGAAGGAGGGGTCGTAACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 613 |
| LEAP-Seq percent confirming: | 99.5526 |
| LEAP-Seq n confirming: | 1335 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACGTCTGCCTTCTAGTGG |
| Suggested primer 2: | AACATCTGCTCCAAGTCGCT |