| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.216254 |
| Chromosome: | chromosome 12 |
| Location: | 8323949 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549650 | (1 of 89) 3.6.4.13 - RNA helicase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGAAGCGAGTGTCGCAGGCCTTCCGAGT |
| Internal bar code: | CGGAAAAGTGGGCGGCGTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 761 |
| LEAP-Seq percent confirming: | 98.9196 |
| LEAP-Seq n confirming: | 3296 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTACAGTTCTTGGCATCA |
| Suggested primer 2: | TAGGCTGACTGACAACGTGC |