| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216263 |
| Chromosome: | chromosome 2 |
| Location: | 992578 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080300 | (1 of 5) PTHR10791 - RAG1-ACTIVATING PROTEIN 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCGCAGTGCGGCGTAGTAGAGCACCA |
| Internal bar code: | CTAAGATGCCTGCGTTGCGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 313 |
| LEAP-Seq percent confirming: | 99.0175 |
| LEAP-Seq n confirming: | 3225 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCGACATCATGTGCTAC |
| Suggested primer 2: | AGTTCGGATGTGTCCCTGTC |