| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216271 |
| Chromosome: | chromosome 10 |
| Location: | 1355638 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g427850 | (1 of 1) IPR000014//IPR000104//IPR013767 - PAS domain // Antifreeze protein, type I // PAS fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCACCACCAGTGTGCGGCCCGGCCCGCC |
| Internal bar code: | CGCATCGGCGGCGGGCCGTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 532 |
| LEAP-Seq percent confirming: | 99.4258 |
| LEAP-Seq n confirming: | 1039 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCATACGAAGGCTTGACC |
| Suggested primer 2: | CAACAACATGAACGTCCTGG |