| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.216278 |
| Chromosome: | chromosome 6 |
| Location: | 7566183 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g300850 | (1 of 1) PTHR22715//PTHR22715:SF0 - TRANSFORMING GROWTH FACTOR BETA REGULATED GENE 1 // TRANSFORMING GROWTH FACTOR BETA REGULATOR 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAACGAAAGCTACTGGGCGTGTCCAAAG |
| Internal bar code: | CCCACCTCGGCCGGTGGTATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 451 |
| LEAP-Seq percent confirming: | 99.8082 |
| LEAP-Seq n confirming: | 1041 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAACCAAACCTGCTCCAA |
| Suggested primer 2: | AACATGACCCTGCCTGAAAC |