| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.216281 |
| Chromosome: | chromosome 16 |
| Location: | 2194363 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g658400 | FDX2 | Apoferredoxin; (1 of 6) K02639 - ferredoxin (petF) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGCGCTTACTACCCAGACACAGCATGT |
| Internal bar code: | CTATGTGGGTCCAATCGTATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 536 |
| LEAP-Seq percent confirming: | 99.735 |
| LEAP-Seq n confirming: | 4892 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGAAGGCCTCAGGTCAA |
| Suggested primer 2: | ATGGACGAGGATGACTGAGG |