| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.216292 |
| Chromosome: | chromosome 10 |
| Location: | 633982 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422000 | TMG6,TRM1 | (1 of 1) PTHR10631:SF3 - TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE; tRNA (guanine-N2)-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTTTGCCTGCCCCTCCTGCAGCACCTTG |
| Internal bar code: | TCCACACCGGCCTGTCAAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 651 |
| LEAP-Seq percent confirming: | 98.939 |
| LEAP-Seq n confirming: | 373 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGAACACACCTGAACCC |
| Suggested primer 2: | TAAGCTTGCCGTTGGAGAGT |