| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.216302 |
| Chromosome: | chromosome 12 |
| Location: | 354193 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g494350 | EMP70 | Endomembrane protein 70; (1 of 1) PTHR10766:SF54 - ENDOMEMBRANE FAMILY PROTEIN 70 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGATGGGGGAGGGGGAGGGAGGCAGGC |
| Internal bar code: | ATTAGACCCGGTCGCGGTTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 539 |
| LEAP-Seq percent confirming: | 99.5268 |
| LEAP-Seq n confirming: | 1893 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGAGAACCGGGAGAAGT |
| Suggested primer 2: | AGGACGTGGTAAACCATCCA |