| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.216305 |
| Chromosome: | chromosome 7 |
| Location: | 4004441 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g339900 | MAPKKK5 | Mitogen-Activated Protein Kinase Kinase Kinase; (1 of 18) 2.7.10.2//2.7.11.1 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGAGCTGGGAGCTCTGGTGAAGGAGCT |
| Internal bar code: | TTTGGGGGGACCGCTGTGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 631 |
| LEAP-Seq percent confirming: | 99.4271 |
| LEAP-Seq n confirming: | 4686 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGCGCTACTTTCTATGAC |
| Suggested primer 2: | GCATCAATGGCGATAAGGAT |