| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216313 |
| Chromosome: | chromosome_5 |
| Location: | 996743 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g246650 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCGCGGGATTGAGATGTGGAAAGCATGGAA |
| Internal bar code: | GCGTACCTAGGTGGGATGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1010 |
| LEAP-Seq percent confirming: | 99.6616 |
| LEAP-Seq n confirming: | 3829 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTCAGTTGGGGGCTATC |
| Suggested primer 2: | AGTCTGGACAGATTGCGCTT |