| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.216376 |
| Chromosome: | chromosome 9 |
| Location: | 155707 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386734 | (1 of 2) PTHR31646//PTHR31646:SF1 - FAMILY NOT NAMED // ALPHA-1,2-MANNOSYLTRANSFERASE MNN2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGCGGGCAGCGCCAAGTCCCACTTCTC |
| Internal bar code: | CTGTAGCACGAGCAAAGAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1363 |
| LEAP-Seq percent confirming: | 98.9707 |
| LEAP-Seq n confirming: | 1250 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCAAAGAAAGCAACACC |
| Suggested primer 2: | GTCACTTCTTGCCCTTGAGC |