| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.216388 |
| Chromosome: | chromosome 9 |
| Location: | 1644484 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397100 | (1 of 39) IPR009003 - Peptidase S1, PA clan | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCGCAGTAGGCGTCGGCGCGGCTTCCG |
| Internal bar code: | CGGTCAATGGTGAGACGAATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 128 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCCTAGACAGTCCATAGC |
| Suggested primer 2: | ATTGCGAAGCTCTTGACGTT |