| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.216412 |
| Chromosome: | chromosome 16 |
| Location: | 1782603 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g655150 | (1 of 2) PTHR14136:SF11 - THYLAKOID LUMENAL 17.4 KDA PROTEIN, CHLOROPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCTCCCAAGCGAGCTCCTGCCCCAACGA |
| Internal bar code: | CGTATGGCACCTTGCTCCCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 886 |
| LEAP-Seq percent confirming: | 98.3471 |
| LEAP-Seq n confirming: | 1904 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCATCTTGCCCACTACCGT |
| Suggested primer 2: | CAGGTTCAGAGAGAGCCCAC |