| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.216422 |
| Chromosome: | chromosome 6 |
| Location: | 7918478 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g303350 | FAL9 | Conserved protein of unknown function | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTGTCCAGTAACCTGCAGGAGTTCAGC |
| Internal bar code: | GGTCGGTTTTATCGCAGACCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 416 |
| LEAP-Seq percent confirming: | 99.6072 |
| LEAP-Seq n confirming: | 1775 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAAGAGTGACGCAGCATT |
| Suggested primer 2: | TGCTGTGATAGGTGGCAGAG |