Insertion junction: LMJ.RY0402.216429_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.216429
Chromosome:chromosome 8
Location:4680076
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre08.g383702 UBC38 (1 of 2) K10586 - baculoviral IAP repeat-containing protein 6 (apollon) (BIRC6, BRUCE); E2 ubiquitin conjugating enzyme 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCAGCGCCTCAAGTGGTGGCCGCGATGA
Internal bar code:ACGCTCCGCTGGCATGTAAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:328
LEAP-Seq percent confirming:33.913
LEAP-Seq n confirming:117
LEAP-Seq n nonconfirming:228
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTTCAAACACTGCCCCAACT
Suggested primer 2:CTGTTTCCACGGTTTCCATT