Insertion junction: LMJ.RY0402.216439_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.216439
Chromosome:chromosome 3
Location:7390967
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g206200 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATAAAGCTTGTTTGTTCACGAGTCTGCTGC
Internal bar code:GGTTATTGAGAAAACTGTGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:737
LEAP-Seq percent confirming:95.0675
LEAP-Seq n confirming:3026
LEAP-Seq n nonconfirming:157
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GCTAGCGTTTCCTGCGTATC
Suggested primer 2:TTATGATCGGGGCAAGAGAC