| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.216441 |
| Chromosome: | chromosome 9 |
| Location: | 877065 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401551 | BHD1 | 3-beta hydroxysteroid dehydrogenase/isomerase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTCTAGAGCCCGCCACGAGCTTGGTTGG |
| Internal bar code: | TGATGACGGTTAGATTACGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1062 |
| LEAP-Seq percent confirming: | 99.4302 |
| LEAP-Seq n confirming: | 1047 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCAACAAATCCTCGCAAC |
| Suggested primer 2: | GTTATATGGCCCAATGCCAC |