| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216443 |
| Chromosome: | chromosome 10 |
| Location: | 5996639 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462750 | (1 of 1) K11374 - elongator complex protein 2 (ELP2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTGCACTACCTGACACACACATGTATG |
| Internal bar code: | CCATGGGCGGCTCTGGCCCGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 594 |
| LEAP-Seq percent confirming: | 44.1241 |
| LEAP-Seq n confirming: | 4990 |
| LEAP-Seq n nonconfirming: | 6319 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGCACACATGCACACAC |
| Suggested primer 2: | GGTGAAGAATGGGGAATGTG |