| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216448 |
| Chromosome: | chromosome 12 |
| Location: | 735289 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g495250 | FAL15,FAP31 | TPR-Repeat Flagellar Associated Protein 31; (1 of 14) PF13424 - Tetratricopeptide repeat (TPR_12) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCGCTTCCACCGCGGTAGAAGTCTCGG |
| Internal bar code: | GGGTCTGGGGGCGGCTACTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 339 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 156 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGAGCTGGCCGAAAACACT |
| Suggested primer 2: | AGGACTCCAGGAAGGAGAGC |