| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216458 |
| Chromosome: | chromosome 6 |
| Location: | 1815909 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g262650 | OPR22,TAA1 | (1 of 66) 2.7.12.1 - Dual-specificity kinase; Translation factor for psaA | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGCCCCTCACTGCGGCTGCGACCGACT |
| Internal bar code: | GGGTAGGGCATCTTTTTGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 74 |
| LEAP-Seq percent confirming: | 54.4444 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCACACGCTTTCACCATGT |
| Suggested primer 2: | GAATACGCGTAGGACCGTGT |