Insertion junction: LMJ.RY0402.216498_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.216498
Chromosome:chromosome 17
Location:3201536
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g722150 PKS3 (1 of 1) 2.3.1.119//2.3.1.199 - Transferred entry: 1.1.1.330, 1.3.1.93, 2.3.1.199 and 4.2.1.134 // Very-long-chain 3-oxoacyl-CoA synthase / Very-long-chain beta-ketoacyl-CoA synthase; Type III polyketide synthase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCACAGTACCCTCCCCGCGCGGAATGGT
Internal bar code:GGCGGCTTCCTCCGAACCCCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:221
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:ATGAGGAAACAGGCTGTGCT
Suggested primer 2:CACTTGCATGGTGGGTACTG