Insertion junction: LMJ.RY0402.216498_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.216498
Chromosome:chromosome_17
Location:3201537
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre17.g722150 PKS3 Type III polyketide synthase antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GAAATGAGAACCTGTCGGGGGATGTTAGAT
Internal bar code:GTGGACCGTAACCCGTTTGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:486
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:228
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CACTTGCATGGTGGGTACTG
Suggested primer 2:ATGAGGAAACAGGCTGTGCT