| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216502 |
| Chromosome: | chromosome 12 |
| Location: | 7735788 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553850 | TUL1 | (1 of 781) IPR000104 - Antifreeze protein, type I; Tubulin-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACGTTTGACCTGTCACACACTTTCGGCG |
| Internal bar code: | GGTCGCGGGCCTCGCACCAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1036 |
| LEAP-Seq percent confirming: | 99.8245 |
| LEAP-Seq n confirming: | 8533 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGACATTGTCGTTTCCCT |
| Suggested primer 2: | ACGTTCGGTGGTCTATGGAG |