| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.216503 |
| Chromosome: | chromosome 1 |
| Location: | 835505 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004300 | ASNS,ASN1 | (1 of 1) K01953 - asparagine synthase (glutamine-hydrolysing) (asnB, ASNS); Asparagine synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGCGTTGTGCTTGTCACTTCCCAGTACA |
| Internal bar code: | CATGTACCTTGCTCTCGCCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 395 |
| LEAP-Seq percent confirming: | 70.1657 |
| LEAP-Seq n confirming: | 127 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACCCAAATGCGTTCACCT |
| Suggested primer 2: | GAAATGGTGCAACAGGAGGT |