Insertion junction: LMJ.RY0402.216517_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.216517
Chromosome:chromosome 12
Location:9004878
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g543700 TNP32 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCATGTCCGTCTCAGCGCTCCCGCACAAC
Internal bar code:TCTGCGAATTAGGAGGGGTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:687
LEAP-Seq percent confirming:99.2841
LEAP-Seq n confirming:1803
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGTCCGCGTGTACATTGA
Suggested primer 2:CATGAGTGACGGTGGTTGAC