Insertion junction: LMJ.RY0402.216530_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.216530
Chromosome:chromosome 10
Location:5414823
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g458350 SRR16 (1 of 1) PTHR22803//PTHR22803:SF55 - MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED // C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN 162; Scavenger receptor cysteine rich (SRCR) protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCATGTACAATTGCGTGATTGTGCATCA
Internal bar code:ATCCGATCTAGTTAGATGAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:324
LEAP-Seq percent confirming:95.7783
LEAP-Seq n confirming:4424
LEAP-Seq n nonconfirming:195
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AAGACATCGTGCGTGACAAG
Suggested primer 2:TTACGGCCTTTCAACATTCC