| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216544 |
| Chromosome: | chromosome 16 |
| Location: | 7128080 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680566 | (1 of 2) K17506 - protein phosphatase 1L [EC:3.1.3.16] (PPM1L, PP2CE) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGTTCCCATCTTGGGGCCACTGTCTCCA |
| Internal bar code: | TGCTGTGCTCTCATCCTCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 857 |
| LEAP-Seq percent confirming: | 99.4236 |
| LEAP-Seq n confirming: | 690 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGAGGGCTAGCGATGAG |
| Suggested primer 2: | CATTTTGGGGTGATGGTTTC |