| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.216546 |
| Chromosome: | chromosome 3 |
| Location: | 1726693 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g153600 | (1 of 1) IPR000104//IPR003034//IPR022656 - Antifreeze protein, type I // SAP domain // XPA C- terminal | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCACATGTTGGCAGTGCTGCTGTTGCTGA |
| Internal bar code: | ATCCAGGGTGCATGTCGACACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 474 |
| LEAP-Seq percent confirming: | 98.9247 |
| LEAP-Seq n confirming: | 1012 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGGTTTGAGTTCCTGCTC |
| Suggested primer 2: | GCTAAGTGGGGCTGCTGTAG |