| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.216548 |
| Chromosome: | chromosome 2 |
| Location: | 2299899 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g090550 | ARS9 | Arylsulfatase; (1 of 1) PTHR10342//PTHR10342:SF228 - ARYLSULFATASE // EXTRACELLULAR SULFATASE SULF-1 HOMOLOG | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGTAGGGGCAGGGGGGTAGCTGTTGAT |
| Internal bar code: | GCGCCAGAGGTTTCTGAAATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 800 |
| LEAP-Seq percent confirming: | 87.931 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTCCTCCGTGTGCTTTGT |
| Suggested primer 2: | CTGGAGCCATGGGAGTATGT |