| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.216551 |
| Chromosome: | chromosome 13 |
| Location: | 4510172 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g602500 | (1 of 1) PF13772 - AIG2-like family (AIG2_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAATCCCACGCGGCGTCGCTGCTAGGCT |
| Internal bar code: | TACGCCCCGTGAGGGGCGCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 790 |
| LEAP-Seq percent confirming: | 99.8743 |
| LEAP-Seq n confirming: | 2384 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCGACATTTTGCTACTGC |
| Suggested primer 2: | ACAAGTGGCGTGTGTAGCAG |