Insertion junction: LMJ.RY0402.216559_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.216559
Chromosome:chromosome 1
Location:4703120
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g032450 (1 of 4) K06816 - golgi apparatus protein 1 (GLG1, ESL1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCTCAAACACCCCCACTCTCACCTTGTC
Internal bar code:CGGGGATTTGTGTAGGGGTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:908
LEAP-Seq percent confirming:99.6785
LEAP-Seq n confirming:930
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGTTGAAACCGTCACCAT
Suggested primer 2:TACTCGCTCAACTACCGCCT