| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.216567 |
| Chromosome: | chromosome 3 |
| Location: | 5164898 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182200 | (1 of 1) IPR001054//IPR028082//IPR029787 - Adenylyl cyclase class-3/4/guanylyl cyclase // Periplasmic binding protein-like I // Nucleotide cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGAAGCCCAGCGCCGACACGACGCCGCT |
| Internal bar code: | GTTCGGAAGGTCGCTAGTGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 430 |
| LEAP-Seq percent confirming: | 98.3333 |
| LEAP-Seq n confirming: | 118 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGCAGACTCCTTCACCA |
| Suggested primer 2: | GAATGCCTGCACTAAGCACA |