| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.216584 |
| Chromosome: | chromosome 6 |
| Location: | 4199032 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278234 | FAP343 | (1 of 19) PF00397 - WW domain (WW); Flagellar Associated Protein 343 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGCGTACAGCGAGCACCCTACCACCTG |
| Internal bar code: | GGTACTACTACTTGCTACTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 656 |
| LEAP-Seq percent confirming: | 98.9274 |
| LEAP-Seq n confirming: | 13097 |
| LEAP-Seq n nonconfirming: | 142 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCCACACAGAAACACGTA |
| Suggested primer 2: | ACTCGTATTGCTGGGGATTG |