| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.216600 |
| Chromosome: | chromosome 5 |
| Location: | 2864091 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g237910 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATTCCGCGGCGCCCTGATCTTATCTACT |
| Internal bar code: | TCATTGGGTCGAGAGAAGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 878 |
| LEAP-Seq percent confirming: | 74.4526 |
| LEAP-Seq n confirming: | 102 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACCCCCTTGGCTATGTA |
| Suggested primer 2: | GCGTGGAGTACCTTGTGGAT |